Part:BBa_K136064

fliL promoter + WT RBS ( K136009) - EnvZ*

EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.

fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).

Its promoter is consitutively repressed.

It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

This construction may allow, with the add of a reporter tag, to see some oscillations

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 476
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 476
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 476
  Illegal BglII site found at 396
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 476
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 476
  
• 1000
  COMPATIBLE WITH RFC[1000]